cytotoxic effects of some 1-[(benzofuran-2-yl)-phenylmethyl]-imidazoles on mcf-7 and hela cell lines
Authors
abstract
aromatase inhibitors have been used as a second line therapy after tamoxifen, in estrogen-receptor positive (er+) women to reduce estrogen concentration in plasma and breast tumors. it has been shown that benzofuran(phenylmethyl)imidazoles are potent aromatase inhibitors at nano-molar concentrations, about 1000 times more potent than aminoglutetimide. in this study, cytotoxic activity of some of these potent aromatase inhibitors (compounds 1-6) on mcf-7 and hela cell lines were evaluated. mcf-7 and hela cells were cultured on rpmi medium and the cells obtained after the third generation were used in this study. the cytotoxic activity of the compounds was first screened at 20 and 100 µm. the ic50 values were then determined, using mtt method. briefly, after 24 h incubation of the cells, the compounds, doxorubicine and the medium were added to the cells and incubated for further 48 h (37 oc, 5% co2). the mtt solution was then added and the absorbance was measured by elisa plate reader at 540 nm after 4 h incubation. the results indicated that the ic50 values for compounds 1-6 were around 35-80 µm for mcf-7 and 47-85 µm for hela cells. compound 5, the 4-chloro derivative, showed almost the highest toxicity on both cell lines. i.e. ic50s were 35 µm and 55 µm for mcf-7 and hela cells, respectively. the cytotoxic activity could be in part due to the aromatase inhibition but other mechanisms like dna degradation may also be involved.
similar resources
genotoxic effects of some l-[(benzofuran-2-yl)-phenylmethyl]-imidazoles on mcf-7 cell line
increased exposure to estrogen has been associated with the risk of breast cancer. substituted benzofuran derivatives with inhibitory effects on estrogen synthesis could be considered as a potential approach to reduce the risk of breast cancer. the study of cytotoxic effects of these compounds has suggested involvement of other mechanisms such as dna damage. in the current study we have investi...
full textgenotoxic effects of 2-methoxy and 2-methyl benzofuran phenylmethyl imidazole on mcf-7 cell line
full text
cytotoxic effects of pinus eldarica essential oil and extracts on hela and mcf-7 cell lines
several attempts have so far been made in the search of new anticancer agents of plant origin. some studies have reported that different species of pine genus possess cytotoxic activities against various cancer cell lines. in the present study, we evaluated the cytotoxic effects of pinus eldarica bark and leaf extracts or leaf essential oil on hela and mcf-7 tumor cell lines. hydroalcoholic and...
full textCtotoxic and apoptogenic effects of Perovskia abrotanoides flower extract on MCF-7 and HeLa cell lines
Objective: Perovskia abrotanoides Karel, belongs to the family Lamiaceae and grows wild alongside the mountainous roads inarid and cold climate of Northern Iran. The anti-tumor activity of P. abrotanoides root extract has been shown previously. This study was designed to examine in vitro anti-proliferative and pro-apoptotic effects of flower extract of P. abrotanoides on MCF-7 and Hela cell lin...
full textCytotoxic effects of Pinus eldarica essential oil and extracts on HeLa and MCF-7 cell lines
Several attempts have so far been made in the search of new anticancer agents of plant origin. Some studies have reported that different species of Pine genus possess cytotoxic activities against various cancer cell lines. In the present study, we evaluated the cytotoxic effects of Pinus eldarica bark and leaf extracts or leaf essential oil on HeLa and MCF-7 tumor cell lines. Hydroalcoholic and...
full textThe Cytotoxicity of Dextran-coated Iron Oxide Nanoparticles on Hela and MCF-7 Cancerous Cell Lines
Background: Recently, iron oxide nanoparticles have attracted attention in various diagnosis and treatment fields. The aim of the present study was to investigate the cytotoxicity of various concentrations and incubation times of dextran-coated iron oxide nanoparticles (DIONPs) on HeLa and MCF-7 cancerous cell lines. Methods: This in-vitro study was conducted at Pharmaceutical Sciences Researc...
full textMy Resources
Save resource for easier access later
Journal title:
research in pharmaceutical sciencesجلد ۱، شماره ۲، صفحات ۷۳-۰
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023